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KMID : 0380620130450010133
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Korean Journal of Food Science and Technology
2013 Volume.45 No. 1 p.133 ~ p.136
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Comparison of Conventional Culture Method and Real-time PCR for Detection of Yersinia enterocolitica in Sausage and Vegetable Salad
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Kim Yun-Gyeong
Chon Jung-Whan
Lee Jae-Hoon
Seo Kun-Ho
Kwak Hyo-Sun
Hwang In-Gyun
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Abstract
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The purpose of this study was to compare a conventional culture method and real-time PCR for the detection
of Yersinia enterocolitica (Y. enterocolitica) in sausage and in vegetable salad. Food samples inoculated with Y.
enterocolitica were enriched in peptone-sorbitol bile-broth, and swabs were then streaked onto cefsulodin-irgasannovobiocin
agar. Biochemical tests for suspected colonies were performed with an API 20E strip. In parallel, real-time
PCR was performed, targeting the 16S rRNA gene using 1 mL of enrichment broth. In sausage, the number of positive
samples detected by culture method (49 out of 60) was similar (p>0.05) with that of real-time PCR (50 out of 60).
However, the number of positive samples of real-time PCR (26 out of 60) was significantly higher (p<0.05) than that of
the conventional culture method (6 out of 60) in vegetable salad. Real-time PCR could be an effective screening tool for
detecting Y. enterocolitica, particularly in food samples with high levels of background flora, such as a vegetable salad.
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KEYWORD
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Yersinia enterocolitica, culture method, real-time PCR, food
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